Figure 5

Levels of t-PA, PAI-1 antigen, and PAI-1 activity in medium conditioned by unpassaged PaECs and AoECs from the same individual (n = 6). ECs were grown to confluence in 20% fetal bovine serum, and 24-hour serum-containing conditioned media was prepared. t-PA and PAI-1 levels were measured by ELISA as described in “Materials and Methods” in conditioned media of primary ECs cultured under basal conditions. Black bars represent t-PA antigen levels, light gray bars represent PAI-1 antigen levels/100, and gray bars represent PAI-1 activity values.