Figure 3

Analysis of BH3-only mRNA expression upon exposure to demethyling agent 5′-aza-2′-deoxycytidine. Quantitative real-time RT-PCR in the cell line Caki-2 shows 7.4±0.8-fold increase of Nbk expression upon culture in the presence of the DNA methylation inhibitor 5′-aza-2′-deoxycytidine (5′-aza-2′-dC). Means±S.D. from three independent experiments are shown. Bax, Bak, Bok and mRNA expression of other BH3-only proteins remains largely unaffected. Noxa was undetectable by real-time PCR in Caki-2