Figure 6
Nbk expression induces cytochrome c release and caspase activation in RCC cells. RCC cells were incubated in the presence of medium (M) or the conditional adenoviral Nbk expression vector that contains Nbk under the control of the Tet-off system in the presence (+Dox) or absence of doxycyclin (−Dox). In the presence of doxycyclin (+Dox), the expression is switched off and Nbk expression is switched on in the absence of doxycyclin. Western blot analyses for Nbk expression, cytochrome c release and caspase-3 activation are shown. Arrows indicate Nbk (left row), cytochrome c (middle row), procaspase-3 and processing to the 16 and 18 kDa subunits (right row)
