Table 1 Proportion of quantifiable hairs (‘success rate’) by marker from scalp and eyebrow

From: Plucked human hair as a tissue in which to assess pharmacodynamic end points during drug development studies

 

Plucked

Stained

Through to resin

Lost/damaged

Zero count

High background

Quantified >0 (% of total)

Scalp

 Ki67

40

35

35

6

0

0

34 (85%)

 Total pRb

40

36

32

5

4

0

31 (78%)

 Phospho-pRb

40

37

24

5

14

1

20 (50%)

 Total p27

40

35

30

10

1

0

29 (73%)

 Phospho-p27

40

36

21

10

12

1

17 (43%)

 Phospho-HH3

40

33

27

8

6

10

16 (40%)

 Total

240

212

169

44 (18%)

37 (15%)

12 (5%)

147 (61%)

Eyebrow

 Ki67

20

20

18

2

2

0

16 (80%)

 Total pRb

20

19

19

1

0

0

19 (95%)

 Phospho-pRb

20

20

13

0

7

2

11 (55%)

 Total p27

20

20

8

1

11

1

7 (35%)

 Phospho-p27

20

20

4

0

20

0

0 (0%)

 Phospho-HH3

20

14

10

8

6

4

2 (10%)

 Total

120

113

72

12 (10%)

46 (38%)

7 (6%)

55 (46%)

  1. Phospho-p27=phosphorylated p27; phospho-pRb=phosphorylated pRb; phospho-HH3=phosphorylated histone H3.
  2. The numbers of hairs in the four columns on the right-hand side add up to the total number of hairs plucked. Hairs could be registered as lost/damaged, or quantified as having no staining, either before or after being taken through to resin.
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