Figure 6
Subcellular distributions of α1-adrenergic receptor subtypes and the effect of charcoal/dextran on α1D-adrenergic receptor localization, as well as intracellular Ca2+ response to phenylephrine in cultured rat myocytes. (A and B) Immunofluorescent images of freshly dispersed VSMCs. VSMCs cultured for >2 days in the absence or presence of charcoal/dextran (-C/D or +C/D), and NRVM cultured for 2 days stained with BODIPY FL prazosin (A) or antibodies specific for α1D-ARs or α1A-ARs (B) are shown in green. The nucleus was labeled with 8 μg/mL Hochest (blue). The arrows in panels A and B indicate relatively obvious cell membrane labeling of the antibody. The number for each staining was between 3 and 5 determinations, and the scale bar=10 μm for all images. (C) Typical traces for Ca2+ signal responses to PE in Fluo-4-loaded myocytes of each group. Numbers for each curve were from 4 to 7 separate experiments.
