Figure 4
From: Melatonin induces transcriptional regulation of Bim by FoxO3a in HepG2 cells
Induction of FoxO3a nuclear translocation and Bim promoter occupancy after melatonin treatment. (A) FoxO3a nuclear translocation. ***P<0.001 significant differences in nuclear localisation of FoxO3a in control vs melatonin-treated cells. Data points represent mean±s.d. from separate high-power field images. Bar=10 μ m. (B) Effect of melatonin on FoxO3a cytoplasmic and nuclear protein expression. Lower panel is nuclear to cytoplasmic ratios of FoxO3a of western blot samples. (C) Effect of FoxO3a silencing and melatonin treatment on Bim expression. (D) Melatonin enhances binding of FoxO3a to Bim promoter region as analysed by ChIP. (E) Effect of FoxO3a and Bim silencing on HepG2 cell viability. Representative results of three individual experiments. *P<0.05. Abbreviations: a. u.=arbitrary units; IgG=rabbit control immunoglobulin G; NTC=non-template control; RLU=relative light unit.
