Figure 3 | British Journal of Cancer

Figure 3

From: Patient-derived glioblastoma cells show significant heterogeneity in treatment responses to the inhibitor-of-apoptosis-protein antagonist birinapant

Figure 3

Type A response pattern in TMZ-sensitive U251 cells. (A–D) Flow cytometry analysis identified a significant apoptotic and secondary necrotic cell death in response to TMZ but not birinapant, with partial sensitivity to zVAD treatment. (E, F) Combined treatment with birinapant further enhanced cell death, a finding also confirmed by morphological analysis of nuclear condensation. Two-way ANOVA, *P<0.05, **P<0.01, ***P<0.001 versus control cells. Scale bar, 50 μm. (G) Protein expressions show that the TMZ alone and in combination accelerate caspase-8 cleavage and induced reduction of RIP1 and further increased of FADD expression. Actin was used as a loading control.

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