Figure 5 | British Journal of Cancer

Figure 5

From: Patient-derived glioblastoma cells show significant heterogeneity in treatment responses to the inhibitor-of-apoptosis-protein antagonist birinapant

Figure 5

Type C response pattern in RN1luc cells. (A–D) Flow cytometry analysis showed moderate sensitisation with the combined treatment. Cell death was not entirely blocked by zVAD. (E, F) A similar finding was observed by Hoechst staining. Two-way ANOVA, *P<0.05 versus control cells, +P<0.05 versus treated cells. Scale bar, 50 μm. (G) Western blot analysis demonstrated a loss of RIP1 expression following birinapant treatment but addition of TMZ reversed the effect of birinapant. Neither single nor combination treatment induced any loss of FADD expression nor cleavage of caspase-8. Actin was used as a loading control.

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