Figure 1 | Cell Death & Disease

Figure 1

From: Direct regulation of FOXK1 by C-jun promotes proliferation, invasion and metastasis in gastric cancer cells

Figure 1

FOXK1 facilitates the malignant biological behavior of GC cells. (A) Proteins isolated from resected tumor and adjacent non-tumor tissue specimens were subjected to western blot analysis. T, GC tissues: N, normal tissues. β-Actin was used as the internal control. (B) FOXK1 expression in normal (a) or cancerous gastric tissue specimens (b and c) was detected using immunohistochemical (IHC) assays. (C) FOXK1 expression was detected using western blot analysis. (D) The proliferation of stable FOXK1 transfectants and vector was evaluated using a WST-8 assay. ****P<0.0001. (E) DNA synthesis of BCR823 cells was measured using an EdU (5-ethynyl-2′-deoxyuridine) incorporation assay after the indicated transfection at 48 h. ****P<0.0001, between FOXK1 and vector. (F and G) FOXK1 overexpression significantly promoted migration and invasive ability compared with vector. ****P<0.0001. All experiments were repeated three times with identical findings. Scale bars, 100 μm in (b and e)

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