Figure 1
From: NDRG1 inhibition sensitizes osteosarcoma cells to combretastatin A-4 through targeting autophagy
CA-4 induces autophagy in OS cells. (a) SJSA and MG63.2 cells were treated with various concentrations of CA-4, and the whole-cell lysates were subjected to immunoblotting of LC3B-II, SQSTM1 and GAPDH. (b and c) The protein bands in a were quantified, and the LC3-II/GAPDH and SQSTM1/GAPDH ratios were calculated and displayed. (d) SJSA and MG63.2 cells were treated with CA-4 in the presence or absence of CQ, and the whole-cell lysates were subjected to immunoblotting of LC3B-II, SQSTM1 and GAPDH. (e and f) The protein bands in d were quantified, and the LC3-II/GAPDH and SQSTM1/GAPDH ratios were calculated. (g) SJSA and MG63.2 cells expressing GFP-LC3 were treated with control, CA-4 and CQ, and the GFP-LC3 puncta were observed under confocal microscopy. (h) Quantification of the number of GFP-LC3 puncta per cell in (g). The data were presented as mean±S.D (*P<0.05, n=3)
