Figure 4
From: MiR-629 promotes human pancreatic cancer progression by targeting FOXO3
MiR-629 inhibition promotes CSC-like traits in pancreatic cancer cells. (a) Distribution of CD44+CD24+SEA+ cells using a flow cytometric analysis. The experiment was repeated three times (*P<0.05). (b) Real-time RT-PCR analysis of mRNA expression that miR-629 overexpression significantly upregulated the mRNA expression levels of stem cell markers, including CyclinD1, ABCG2, OCT4, SOX2, and NANOG, but downregulated the mRNA expression levels of multiple pluripotency factors, including FOXO3, p21, and p27, in the indicated cells. Transcript levels were normalized to GAPDH expression. Error bars represent the mean±S.D. of three independent experiments (*P<0.05)
