Figure 3 | Experimental & Molecular Medicine

Figure 3

From: Histone deacetylase inhibitor KBH-A42 inhibits cytokine production in RAW 264.7 macrophage cells and in vivo endotoxemia model

Figure 3

(A) mRNA levels of iNOS, TNF-α, IL-1β and IL-6 by RT-PCR (RAW 264.7 macrophage cells). HDAC inhibitor, KBH-A42 was treated 1h before LPS stimulation. Total RNA's of RAW 264.7 cells were isolated 6 h after LPS stimulation (200 ng/ml) and RT-PCR was performed on iNOS, TNF-α, IL-1β, IL-6 and β-actin mRNA's. (B) Densitometric analysis of autoradiographic signals corresponding to iNOS, THF-α, IL-1β, IL-6 mRNAs corrected on the basis of β-actin mRNA signals in the same lane.

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