Figure 3 | Experimental & Molecular Medicine

Figure 3

From: Transcriptional induction of DLC-1 gene through Sp1 sites by histone deacetylase inhibitors in gastric cancer cells

Figure 3

Identification of the Sp1 binding sites responsible for the activation of DLC-1 promoter by TSA. (A) Sequences of the DLC-1 promoter containing the region -219/-174 bp. The consensus sequences for Sp1 binding sites (GGCGGG or GGGCGG) are underlined. (B) Base substitution mutants using -219/+117 as a template were constructed as shown in the left panel. Theses constructs were transiently transfected into SNU-5 and SNU-668 cells followed by treatment with DMSO or 330 nM TSA for 12 h. Luciferase activity was measured and normalized to protein concentration in all luciferase reporter experiments. The data represent the combined results of three independent experiments performed in triplicate.

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