Figure 4 | Experimental & Molecular Medicine

Figure 4

From: The N- and C-terminal domains of parathyroid hormone-related protein affect differently the osteogenic and adipogenic potential of human mesenchymal stem cells

Figure 4

Effect of PTHrP (1-36) and PTHrP (107-139) on commitment of human MSCs to adipocytic lineage. MSC cultures in adipogenic medium (AM) were exposed to 10 nM of PTHrP (1-36) or PTHrP (107-139) from either day one or day six. (A) Gene expression of PPAR-γ and LPL at different times of cell exposure to each PTHrP peptide or vehicle. (B) Percentage of adipocytes and spectrophotometric quantification of oil red staining of fat vesicles in MSCs at day 24 after adipogenic induction. (C) Representative cultures induced to adipocytes and stained with oil red, treated or untreated with PTHrP (1-36) or PTHrP (107-139) are shown (magnification, × 200). Values are mean ± SEM of 3 different cultures, each from a different subject, analyzed in duplicate. The mRNA expression is expressed as a fold change relative to day 6 untreated MSC adipocyte induced as a reference (value = 1). *P < 0.05 vs corresponding peptide-untreated value. †P < 0.05 vs corresponding PTHrP (1-36)-treated value.

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