Figure 1 | Experimental & Molecular Medicine

Figure 1

From: Nuclear factor of activated T cells negatively regulates expression of the tumor necrosis factor receptor-related 2 gene in T cells

Figure 1

Effect of CsA on TR2 gene expression in primary murine CD4+ T cells and the EL-4 T cell line. Purified CD4+ T or EL-4 T cells were incubated in the presence or absence of cyclosporin A (400 ng/ml), and TR2 expression was assessed by FACS or RT-PCR. (A) Murine CD4+ T cells or (B) EL-4 cells were stained with anti-TR2 mAb (12H9, rat IgG2b) and FITC-conjugated secondary antibodies, and levels of TR2 expression were determined by FACS analysis. Negative controls with isotypic-matched antibody were indicated in thick line (A) or shaded histogram (B). (C) Total RNA was prepared from EL-4 cells and analyzed by reverse transcript-PCR using mTR2 (27 cycles) and GAPDH (30 cycles) primer pairs in non-saturating conditions. (D) Expression of TR2 protein was determined by Western blot analysis.

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