Figure 3

BLT2 D196G leads to enhanced migration under low-dose ligand stimulation. (a–d) CHO-K1 cells were seeded onto 6-well culture plates and then were transiently transfected with BLT2 or BLT2 D196G. Cells were then incubated in a standard CO₂ incubator to 80% confluency, and then wounds were generated with a 200p pipette tip. (a) The wounded cells were washed twice and then were treated with 10 nM of the indicated ligand-containing RPMI medium with 1% FBS. Photographs of the cultures were obtained immediately after scratching and at 24 h later in the same position. (b–d) The dose dependence of migration was determined after treatment with the indicated ligands, as described above. The results are expressed as a fold-change relative to 0 nM of each ligand, and the data are represented as means±s.d.; n=3 independent experiments. *P<0.01 and **P<0.005 according to Student’s t-test. The t-test was performed to determine whether the difference was significant between BLT2 and BLT2 D196G with a ligand concentration of 10 nM.