Figure 5

Wnt11 regulation of zone-3 signature genes through a non-canonical pathway. (a) RNA extracted from mouse primary hepatocytes treated with phosphate-buffered saline (PBS), Wnt11 (50 ng ml⁻¹) or GSK3β inhibitor (5 μM) was subjected to quantitative RT-PCR array of zone-3 genes. The heat map represents the relative abundance of the indicated genes. The scale bar indicates the expression change in fold index. (b) Huh7 cells were cotransfected with TOPFLASH, a firefly luciferase reporter regulated by β-Catenin, and a renilla luciferase vector. Sixteen hours after transfection, cells were treated with the indicated ligands for 20 h followed by a dual luciferase assay. The relative intensity of canonical Wnt signaling activity is shown as TOPFLASH relative luciferase unit (RLU). *P<0.01. (c, d) Huh7 cells were treated with the indicated Wnts (50 ng ml⁻¹) for 24 h followed by immunoblot analysis of the indicated protein.