Figure 6

Type I IFN receptor signaling enables CD64⁺ cDC generation. (a) Scatter plots of whole-gene expression the DC subsets. The average gene expression of duplicates of each DC subset is shown. The numbers of genes showing <1.5-fold difference in expression between subsets are shown in black. The genes showing ⩾2-fold difference in expression are numbered and color-matched with the relevant DC subsets. RNAs were analyzed from biological duplicates of each DC subset. There were more genes showing ⩾2-fold differences in expression when we compared each cDC subset to the CD64⁺ moDCs, versus comparing the cDC subsets to one another. (b) Heat map of differentially expressed genes (those whose expression levels showed a ⩾3-fold change between cell types and a <3-fold difference between duplicates). Genes were grouped into six clusters and are listed along with their gene ontologies in Supplementary Table 2. (c and d) Wild-type (WT) or Batf3^−/− mice were infected with 2 × 10³ Listeria and popliteal lymph nodes (LNs) were collected at day 3 (c) or day 1 and 2 (d) after infection. (c) Cells were analyzed by flow cytometry. (d) Viable bacteria were quantified. Each symbol represents an individual mouse; horizontal lines indicate the mean (±s.e.m. of N=3 mice per group). (e–g) WT or Ifnar^−/− mice were infected with 2 × 10³ Listeria and popliteal LNs were collected at day 2 (e and f) or day 1 and 2 (g) after infection. (e and f) Cells were analyzed by flow cytometry. (e) The cell numbers of CD64⁺ moDCs and 7/4⁻CD64⁺ cells within the LNs are shown in the graphs. (f) The sum of CD64⁻ cDCs (blue bar) and CD64⁺ cDCs (red bar) cell numbers is shown in the graphs. Mean±s.e.m. of N=4 mice per group. (g) Viable bacteria were quantified. Data are representative of two independent experiments. Each symbol represents an individual mouse; horizontal lines indicate the mean (±s.e.m. of N=3 mice per group).