Table 4 The 50 most significant differentially expressed genes in E. colia

From: Insight into synergetic mechanisms of tetracycline and the selective serotonin reuptake inhibitor, sertraline, in a tetracycline-resistant strain of Escherichia coli

Relative expressions of mRNA

Description (functional group)

Treatment

  

Ser+Tet (8 mg l−1+4 mg l−1)

Tet (32 mg l−1)

Ser (16 mg l−1)

Downregulated

 ridA

Reactive intermediate/imine deaminase (V)

−5.57

0.05

0.90

 yghZ

Aldo/keto reductase (−)

−3.41

0.14

−0.10

 osmY

Hypothetical protein (S)

−4.77

0.53

0.11

 malP

Maltodextrin phosphorylase (G)

−4.11

−1.08

−2.58

 elaB

Protein ElaB (J)

−4.15

−1.58

−1.27

 dhnA

Fructose-bisphosphate aldolase (G)

−5.13

−3.12

−0.73

 lacZ

Beta-d-galactosidase (G)

−6.69

0.17

−0.55

 celF

Alpha-glucosidase/alpha-galactosidase (G)

−5.53

0.13

−0.63

 malQ

4-alpha-glucanotransferase (G)

−4.77

−0.92

−2.29

 NA

Hypothetical protein (−)

−5.97

−5.38

−4.86

 brf

Bacterioferritin (P)

−4.46

−2.11

−1.73

 pduW

Propionate kinase (C)

−6.40

0.38

1.16

 hchA

Chaperone protein HchA (R)

−4.44

−2.02

−1.80

 ppsA

Phosphoenolpyruvate synthase (G)

−3.79

−0.41

0.57

 NA

Hypothetical protein (−)

−5.00

−0.98

−2.01

 wrbA

NAD(P)H:quinone oxidoreductase (C)

−5.00

−0.90

−1.98

 lamB

Maltoporin (G)

−6.92

−1.05

−6.91

 ilvA

Threonine dehydratase (E)

−6.01

0.22

−0.92

 tdcE

PFL-like enzyme TdcE (C)

−5.55

−0.08

1.22

 sdaC

HAAAP family serine/threonine permease (E)

−5.93

0.45

1.55

 gadA

Glutamate decarboxylase (E)

−5.43

−5.19

−3.48

 malE

Maltose ABC transporter substrate-binding protein (G)

−6.64

−2.02

−5.40

 gadC

Glutamate:gamma-aminobutyrate antiporter

−5.71

−4.82

−3.56

 flgL

Flagellin (N)

−6.93

−5.66

−6.98

 NA

Hypothetical protein (−)

−2.77

−0.88

−0.96

Upregulated

 rpoA

DNA-directed RNA polymerase subunit alpha (K)

2.13

0.77

0.06

 rplO

50S ribosomal protein L15 (J)

2.24

0.84

0.27

 rplJ

50S ribosomal protein L10 (J)

2.23

0.45

0.28

 glnH

Glutamine ABC transporter substrate-binding protein (E)

2.53

−0.82

−0.22

 rppL

50S ribosomal protein L16 (J)

2.50

0.32

0.18

 mdh

Malate dehydrogenase (C)

2.15

0.56

1.75

 citF

Citrate lyase subunit alpha (C)

6.63

−1.25

5.58

 typA

GTP-binding protein TypA (T)

2.60

0.38

0.63

 rplK

50S ribosomal protein L11 (J)

2.22

0.45

0.03

 ptaN

Phosphate acetyltransferase (R)

2.38

−1.35

1.18

 glnQ

Glutamine ABC transporter ATP-binding protein (E)

3.23

−0.45

0.19

 priB

Primosomal replication protein N (L)

2.69

0.82

0.47

 rplQ

50S ribosomal protein L17 (J)

2.18

0.97

0.08

 rplA

50S ribosomal protein L1 (J)

2.34

0.55

0.09

 rpsF

30S ribosomal protein S6 (J)

2.77

0.71

0.32

 ackA

Acetate kinase (C)

2.45

−1.08

0.88

 guaB

IMP dehydrogenase (T)

4.23

1.27

1.37

 glnA

Glutamine synthetase (E)

4.25

−0.19

0.82

 pykF

Pyruvate kinase (G)

2.61

−0.73

−0.21

 alkP

2,3-bisphosphoglycerate-independent phosphoglycerate mutase (G)

3.02

−0.67

0.09

 ptsG

PTS glucose-specific subunit IIBC (G)

3.57

−0.28

0.05

 guaA

GMP synthase (E)

3.49

0.18

0.80

 citT

Anion permease (G)

8.31

−0.76

6.49

 asr

Acid-shock protein (−)

8.29

−1.31

8.97

  1. Abbreviations: Ser, sertraline; Tet, tetracycline.
  2. APEC_O2 treated with either Ser or Tet combined Tet and Ser (ST) vs control conditions. The change in expression is relative to control condition, and values are stated as log 2 fold-change. For completeness, the gene regulatory levels of Ser- and Tet-only treatments vs control condition for the same 50 genes are also included in the table. Values in bold are significantly different from control.
Back to article page