Table 6 The 50 most significant differential expressed genes in E. colia

From: Insight into synergetic mechanisms of tetracycline and the selective serotonin reuptake inhibitor, sertraline, in a tetracycline-resistant strain of Escherichia coli

Relative expression of mRNA

Description (functional group) b

Treatment

  

Ser+ Tet (8 μg ml−1+4 μg ml−1)

Tet (32 μg ml−1)

Ser (16 μg ml−1)

Upregulated

 pykF

Pyruvate kinase (G)

2.62

−0.73

−0.20

 ptsG1

PTS glucose-specific subunit IIBC (G)

3.57

−0.28

0.01

 ldcC

Arginine decarboxylase

1.72

−3.05

−3.20

 alkP

Phosphoglycerate mutase (G)

3.02

−0.67

0.08

 NA

Hypothetical protein (S)

−0.19

−0.92

−4.25

 rplA

50S ribosomal protein L1 (J)

2.34

0.55

0.09

 glnH

Glutamine ABC transporter substrate-protein (C)

2.54

−0.83

−0.23

 guaA

GMP synthase (E)

3.50

0.18

0.81

 rplQ

50S ribosomal protein L17 (J)

2.18

0.97

0.08

 rplK

50S ribosomal protein L11 (J)

2.22

0.45

−0.03

 rpsF

30S ribosomal protein S6 (J)

2.77

0.71

0.32

 glnA

Glutamine synthetase (E)

4.25

−0.19

0.82

 glnQ

Glutamine ATP-binding protein (E)

3.23

−0.45

0.19

 rpoA

DNA-directed RNA polymerase subunit alpha (K)

2.13

0.78

0.05

 rpsQ

30S ribosomal protein S17 (J)

2.16

0.43

0.02

 rpsD

30S ribosomal protein S4 (J)

1.97

0.64

0.00

 rppL

50S ribosomal protein L16 (J)

2.50

0.32

0.18

 potE

Arginine:agmatine antiporter (E)

1.53

−3.25

−3.74

 hisJ

Amino-acid transporter (E)

2.04

−0.44

−0.80

 NA

50S ribosomal protein L29 (J)

2.56

0.47

0.18

 araC

Transcriptional regulator (K)

2.25

−1.20

−2.15

 rpsM

30S ribosomal protein S13 (J)

1.95

0.54

−0.03

 pgk

Phosphoglycerate kinase (G)

1.80

−0.54

−0.32

 rplJ

50S ribosomal protein L10 (J)

2.33

0.45

0.28

 rpsK

30S ribosomal protein S11 (J)

2.05

0.74

0.04

 priB

Primosomal replication protein N (L)

2.69

0.82

0.47

Downregulated

 sdaC

HAAAP family serine/threonine permease (E)

−5.94

0.45

1.15

 tdcE

PFL-like enzyme TdcE (C)

−5.54

0.08

1.22

 ilvA

Threonine dehydratase (E)

−6.08

0.21

0.91

 rbsB

ABC transporter substrate-binding protein (G)

−2.64

0.28

3.10

 ackA

Propionate kinase (C)

−6.41

0.38

1.16

 ppsA

Phosphoenolpyruvate synthase (G)

−3.79

−0.41

0.57

 galT

Galactokinase (G)

−2.19

0.92

1.75

 galM

Galactose mutarotase (G)

−1.94

0.72

1.77

 ridA

Reactive intermediate/imine deaminase (−)

−5.58

−0.05

0.90

 csrA

Carbon starvation protein A (−)

−2.57

−0.72

2.09

 glpQ

Glycerophosphoryl diester phosphodiesterase (I)

−0.53

0.87

3.26

 lacZ

Beta-d-galactosidase (G)

−6.70

0.17

−0.55

 nuoG

Oxidoreductase (−)

−3.41

−0.14

−0.10

 celF

Alpha-glucosidase/alpha-galactosidase (G)

−5.53

0.13

−0.62

 ansB

l-asparaginase (J)

−2.07

0.98

1.28

 rbsK

Ribokinase (G)

−0.66

0.14

2.55

 sdaA

Serine dehydratase (E)

−5.45

−0.04

1.24

 dhnA

Fructose-bisphosphate aldolase (G)

−5.13

−3.12

−0.72

 galE

Galactose-1-phosphate uridylyltransferase (G)

−1.64

0.66

1.43

 proP

d-galactose transporter GalP (−)

−2.18

−0.54

0.43

 rbsC

Ribose ABC transporter permease (G)

−1.33

0.47

3.45

 glpT

Glycerol-3-phosphate transporter (G)

2.16

2.37

4.98

 NA

Hypothetical protein (−)

0.09

0.24

2.42

  1. Abbreviations: Ser, sertraline; Tet, tetracycline.
  2. APEC_O2 treated with Ser or Tet or combined Ser and Tet vs individual sertraline treatment. The change in expression is relative to control condition, and values are stated as log 2 fold-change. For completeness, the gene regulatory levels of individually tetracycline treatments vs control condition for the same 50 genes are also included in the table. Values in bold are significantly different from control.
  3. bAccording to KEGG classification: C, energy production and conversion; E, amino-acid transport and metabolism; V, defense mechanism; G, carbohydrate transport and metabolism; P, inorganic transport and metabolism; T, signal-transduction mechanisms; J, translation, ribosomal structure and biogenesis; R, general function prediction only; NA, not available.
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