Figure 5: Effect of sequence depth on coverage and accuracy of human genome sequencing.
From: Accurate whole human genome sequencing using reversible terminator chemistry
ELAND alignments were used for this analysis. a, Accumulation of sequence-based SNP calls, including all SNPs (squares), heterozygous SNPs (triangles) and homozygous SNPs (circles) with increasing input read depth. b, Decrease in genotype positions not covered by sequence (squares), heterozygote under-calls in sequence data relative to genotype data (triangles) and discordant SNP calls compared to genotypes (circles) with increasing input read depth. Vertical dotted lines indicate various input read depths (10×, 15×, 30× haploid genome).
