Extended Data Figure 7: PYR1MANDI is functional in tomato.
From: Agrochemical control of plant water use using engineered abscisic acid receptors
A–D, Transgenic tomato plants expressing the 35S::PYR1MANDI transgene were made as described in the Methods. A single-insert line was obtained that segregated plants with high (H) and low (L) PYR1MANDI protein levels as well as non-transgenic (N, Null) segregants. A, Western blot analyses of SDS–PAGE separated proteins from T2 segregants using an anti-PYR1 antibody. The bottom panel shows the large subunit of Rubisco as a loading control. B, Thermography of representative T2 transgenic plants and wild-type control before and 24 h after treatment with a solution containing 25 μM mandipropamid. The wild-type and H plant images shown in B are the same images shown in Fig. 4. C, Quantification of mean leaf temperatures (measured from the three oldest leaves) before and after mandipropamid treatment. The asterisk indicates a statistically significant (P < 0.05) difference between the high expressing PYR1MANDI line in comparison to the null segregant control, as determined using a two-sided t-test; n = 2 for each of the four sample groups. Error bars show s.d. D, Analysis of an independent primary transgenic tomato PYR1MANDI line. The line was grown alongside a wild-type control and treated with a mock solution and then thermographed 24 h later. Three days after this, the plants were treated with a solution containing 10 μM mandipropamid and thermographed 24 h after application.
