Figure 1: Dynamic reorganization of chromatin structure during differentiation of human ES cells.
From: Chromatin architecture reorganization during stem cell differentiation
a, First principal component (PC1) values and Hi-C interaction heat maps in H1 ES cells and H1-derived lineages. PC1 values are used to determine the A/B compartment status of a given region, where positive PC1 values represent A compartment regions (blue), and negative values represent B compartment regions (yellow). Dashed lines indicate TAD boundaries in ES cells. b, K-means clustering (k = 20) of PC1 values for 40-kb regions of the genome that change A/B compartment status in at least one lineage. c, K-means clustering of PC1 values surrounding TAD boundaries (‘b’ denotes boundary ___location). d, Distribution of fold-change in gene expression for genes that change compartment status (‘A to B’ or ‘B to A’) or that remain the same (‘stable’) upon differentiation (P < 2.2 × 10−16, P values by Wilcoxon test; whiskers correspond to interquartile range). e, Genome browser for two genes of which one (OTX2) shows concordance between expression and PC1 values, whereas a second (TMEM260) does not.
