Extended Data Figure 1: Tomographic map of the human NPC.
From: In situ structural analysis of the human nuclear pore complex
a, Orthoslices through the nucleocytoplasmic axis, CR, IR and NR of a tomographic structure of the human NPC obtained using a direct electron detector (this study) compared to a structure obtained with a conventional detector using a similar experimental workflow (Electron Microscopy Database accession number EMD-2444). In both cases, the CR, IR and NR were aligned independently. The arrowhead indicates a transmembrane ___domain that is resolved in the IR. b, Fourier shell correlation curves of the CR, IR and NR regions. c, Histogram corresponding to the colour-coded local resolution map shown in d that was calculated using ResMap41. d, A single segment of the NR ring is shown (in all other figures the segments are shown jointly with their anterior and/or posterior asymmetric unit) at two different isosurface thresholds. The redundant density of the outer Nup43 β-propeller (horizontal arrowhead) and the Nup133 middle ___domain (vertical arrowhead) of two neighbouring asymmetric units are indicated for orientation. The reduced resolution at the edges is due to the border of the mask used during alignment and averaging that covered about 1.5 times the asymmetric unit.
