Extended Data Figure 1: Anatomical characterization of the AC–CA projection.
From: Projections from neocortex mediate top-down control of memory retrieval
a, Five days after RV-tdT injection into the hippocampus (coordinates specified), retrogradely labelled neurons were detected in the contralateral hippocampus (arrow), medial septum (bracket), and AC (Fig. 1a). Scale bars: ×5: 300 μm; ×10: 100 μm (confocal). b, Eight weeks after injection of CAV-Cre into the hippocampus and DIO-eYFP in the AC (coordinates specified), afferent cell bodies were detected in the AC (arrow). Confocal image; ×10 magnification; scale bar: 100 μm. c, Retrograde tracing with RV-tdT from the AC to map reciprocal connections from the hippocampus. The injection site is indicated in brackets, sparse labelling of afferent cell bodies in left and right hippocampus, primarily in the subiculum (arrows), and also in the medial dorsal thalamic nucleus as expected (asterisk). Confocal ×5, scale bar, 200 μm; ×10 images, scale bar 100 μm.
