Extended Data Figure 2: Engineering and genotyping of A20 OTU mutant and A20 ZnF4 mutant knock-in mice. | Nature

Extended Data Figure 2: Engineering and genotyping of A20 OTU mutant and A20 ZnF4 mutant knock-in mice.

From: Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation

Extended Data Figure 2

a, Schematic diagrams of the A20 protein indicating the locations of the knock-in point mutations for each engineered mouse strain. The gene and protein names are also indicated, with abbreviated protein names indicated in parentheses. b, Tnfaip3Otu/Otu knock-in allele encoding A20 OTU(C103A) and representative genotyping data (right panel). c, Tnfaip3z4Cys/z4Cys knock-in allele encoding A20 ZnF4(C609A,C612A) and representative genotyping data (right panel). d, Tnfaip3z4Ub/z4Ub knock-in allele encoding A20 ZnF4(Y599A,F600A) and representative genotyping data (right panel). b–d, Correctly targeted ES cell clones were identified by long-range PCR followed by sequencing (data not shown). LoxP sites are illustrated as yellow arrows, frt sites as red arrows. Modified exons 3 and 7 are indicated in blue. For gel source data, see Supplementary Fig. 6.

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