Extended Data Figure 6: Genomic DNA analysis of LAIR1-containing antibodies of donor C and donor D.
From: A LAIR1 insertion generates broadly reactive antibodies against malaria variant antigens
a, The sequence alignment of genomic DNA (gDNA) and cDNA of a LAIR1-containing antibody (AB) from donor C (DonC) reveals a 507 bp LAIR1 insert in chromosome 14 (Chr14) and the removal of a 160 bp fragment by RNA splicing. Splice donor and acceptor sites are highlighted in yellow. b, Schematic overview of the genomic organization of a LAIR1-containing antibody from donor D, not to scale. c, Alignment of a region of antibody-encoding DNA (chromosome 14) with the corresponding region of chromosome 13 from gDNA. The sequence maintained in the mature antibody mRNA is boxed and the splice donor site is highlighted in yellow. d, Alignment of gDNA and cDNA reveals that a part of the chromosome 13 region and the entire inserted 5′ LAIR1 intron are removed by RNA splicing. Splice donor and acceptor sites are highlighted in yellow. e, Alignment of the two repeated elements found in the inserted LAIR1 intron in chromosome 14 with the corresponding sequence in chromosome 19. The repeats are named R1 and R2, and K = G/A.
