Extended Data Figure 5: Interaction of the FIP1L1 enhancer with nearby promoters and PDGFRA quantified by reciprocal 3C.
From: Insulator dysfunction and oncogene activation in IDH mutant gliomas
Top, the H3K27ac, CTCF and genetic architecture of the FIP1L1/PDGFRA locus is indicated, highlighting the 3C strategy. Bottom, plots indicate the interaction signal of the indicated sites (black lines) with the common enhancer primer. The FIP1L1 enhancer interacts with local promoters in wild-type and mutant tumours and models. In IDH wild-type gliomas, it shows essentially no interaction with the PDGFRA promoter. In IDH mutant gliomas, it interacts with the PDGFRA promoter with comparable strength to the local interactions, despite the much larger intervening distance (900 kb). Error bars reflect s.d.
