Extended Data Figure 3: β-Arrestin-dependent ERK1/2 phosphorylation.
From: β-Arrestin biosensors reveal a rapid, receptor-dependent activation/deactivation cycle
HEK293 cells were transiently transfected with the indicated constructs or control vector (pcDNA3; Con) and treated without or with isoproterenol for 10 min (10 μM) as indicated. Cell lysates were analysed for pERK1/2 and ERK1/2 by immunoblot analysis. Data represent mean ± s.e.m., n = 6 independent experiments. *P < 0.05 versus unstimulated samples; #P < 0.05 versus isoproterenol-stimulated control (Kruskal–Wallis followed by Mann–Whitney U post-hoc analysis).
