Extended Data Figure 5: Lugdunin activity against eukaryotic cells.
From: Human commensals producing a novel antibiotic impair pathogen colonization
a, b, Human neutrophil granulocytes (a) or erythrocytes (b) were incubated with high concentrations of lugdunin. Their lysis was monitored by the release of the enzyme lactate dehydrogenase (a) or haemoglobin (b), respectively. Cells without lugdunin were used as negative control. Incubation of cells in 2% Triton X-100 was used as positive control for high lysis. The data represent three independent experiments ± s.d. c, Promyelocytic leukaemia (HL60) cells were incubated with high concentrations of lugdunin. In order to determine the 50% inhibitory concentration of lugdunin, the metabolic activity of HL60 cells was measured by the conversion of resazurin into the highly fluorescent resorufin. The apoptosis-inducing staurosporine was used as positive control. IC50 values were calculated from the means of three independent experiments.
