Extended Data Figure 3: Phenotyping of the serum and lungs of Spns2 mice.
From: Genome-wide in vivo screen identifies novel host regulators of metastatic colonization
Sphingoid base levels in the (a) serum (+/+, n = 5; tm1a/tm1a, n = 4) and (b) lungs (+/+, n = 6; tm1a/tm1a, n = 5) of male mice; data are mean ± s.e.m., multiple two-tailed unpaired t-tests with P value adjusted by the Holm–ŠÃdák method with α set to 5%. Sph, sphingosine; DHSph, dihydrosphingosine; S1P, sphingosine-1-phosphate; DHS1P, dihydrosphingosine-1-phosphate. c, Micrograms of extravasated Evans blue dye in the lungs of +/+ and tm1a/tm1a male mice. d, Number of CFSE-labelled B16-F10 cells present in the lungs of female mice 90 min after administration. e, Levels of apoptosis in B16-F10–mCherry cells 12 h after administration to male mice. Shown are representative data from three independent experiments, with symbols representing individual mice. P values are indicated from two-tailed unpaired t-test with Welch’s correction (c–e).
