Extended Data Figure 2: Overview of single-particle cryo-EM for hTRPV6 in nanodiscs. | Nature

Extended Data Figure 2: Overview of single-particle cryo-EM for hTRPV6 in nanodiscs.

From: Opening of the human epithelial calcium channel TRPV6

Extended Data Figure 2

a, Example cryo-EM micrograph for hTRPV6 in nanodiscs with example particles circled in red. b, Orientations of particles that contributed to the final 3.6 Å reconstruction. Longer red rods represent orientations with more particles. c, Local resolution mapped on density at 0.013 threshold level (UCSF Chimera) calculated using Resmap and two unfiltered half maps, with the highest resolution observed for the channel core. d, FSC curve calculated between half maps. e, Cross-validation FSC curves for the refined model versus unfiltered half maps (only half map1 was used for PHENIX refinement) and the unfiltered summed map.

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