Extended Data Figure 1: Characterization of WGBS datasets and validation of Aba–seq method.

a, Distribution of WGBS coverage for each symmetric CpG. For box plots, the upper and lower hinges correspond to the first and third quartiles, the centre line corresponds to the median, and the maxima and minima correspond to the highest and lowest value within 1.5 × the inter-quartile range, respectively. b, Overview of the Aba–seq method¹⁵. c–e, Density heat map showing correlation between levels of 5hmC at all 2-kb windows (minimum of four symmetric CpGs) in E14 mouse ES cells as computed by TAB–seq³⁵ (x-axis) and Aba–seq¹⁵ (y-axis) (c); TAB–seq³⁵ (x-axis) and hMeDIP³⁶ (y-axis) (d) or Aba–seq¹⁵ (x-axis) and hMeDIP³⁶ (y-axis) (e). For c–e, the Pearson correlation coefficient (ρ) is shown. Specific details for all Extended Data Figures regarding sample sizes and how samples were collected can be found in the ‘Statistics and reproducibility’ section.