High–Level ATP Production by a Genetically–Engineered Candida Yeast

1. Picataggio, S., Rohrer, T., Deanda, K., Lanning, D., Reynolds, R., Mielenz, J. and Eirich, L.D. 1992. Metabolic engineering of Candida trapicalis for the production of long-chain dicarboxylic acids. Bio/Technology 10: 894–898.

   CAS  PubMed  Google Scholar 

2. Tani, Y. 1991. Production of useful chemicals by methylotrophs, p. 253–270. In: Biology of Methylotrophs. Goldberg, I. and Rokem, J. S. (Eds.). Butterworth-Heinemann, Boston.

   Chapter  Google Scholar 

3. Goodman, J.M., Trapp, S.B. and Hwang, H. 1990. Peroxisomes induced in Candida boidinii by methanol, oleic acid and D-alanine vary in metabolic function but share common integral membrane proteins. J. Cell Sci. 97: 193–204.

   CAS  PubMed  Google Scholar 

4. Tani, Y., Yonehara, T., Mitani, Y. and Yamada, H. 1984. ATP production by sorbitol-treated cells of a methanol yeast, Candida boidinii (Kioeckera sp.) No 2201. J. Biotechnol. 1: 119–127.

   Article  CAS  Google Scholar 

5. Sakai, Y., Kazarimoto, T. and Tani, Y. 1991. Transformation system for a methylotrophic yeast, Candida boidinii, cloning of the orotidine-5′-phosphate decarboxylase gene (URA3), isolation of uracil auxotrophic mutants, and use of the mutants for integrative transformation. J. Bacteriol. 173: 7458–7463.

   Article  CAS  Google Scholar 

6. Sakai, Y. and Tani, Y. 1992. Cloning and sequencing of alcohol oxidase-encoding gene (AOD1) from the formaldehyde-producing asporogenous methylotrophic yeast, Candida boidinii S2. Gene 114: 67–73.

   Article  CAS  Google Scholar 

7. Konrad, M. 1988. Analysis and in vivo disruption of the gene coding for adenylate kinase (ADK1) in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 263: 19468–19474.

   CAS  PubMed  Google Scholar 

8. Tomasselli, A.G., Mast, E., Janes, W. and Schiltz, E. 1986. The complete amino acid sequence of adenylate kinase from baker's yeast. Eur. J. Biochem. 155: 111–119.

   Article  CAS  Google Scholar 

9. Sakai, Y., Sawai, T. and Tani, Y. 1987. Isolation and characterization of a catabolite repression-insensitive mutant of a methanol yeast, Candida boidinii A5, producing alcohol oxidase in glucose-containing medium. Appl. Environ. Microbiol. 53: 1812–1818.

   CAS  PubMed  PubMed Central  Google Scholar 

10. Tochikura, T. 1978. Production of nucleotide-related substances by yeasts and utilization of fermentation energy to biosynthetic processes—a monograph—(in Japanese). Hakkoukougaku 56: 508–526.

    CAS  Google Scholar 

11. Yamaguchi, S., Fukuda, Y., Shimosaka, M. and Kimura, A. 1984. Phosphorylation of AMP to ATP by dried Escherichia coli B cells. J. Ferment. Technol. 62: 29–33.

    CAS  Google Scholar 

12. Fujio, T. and Furuya, A. 1983. Production of ATP from adenine by Brevibacterium ammoniagenes. J. Ferment. Technol. 61: 261–267.

    CAS  Google Scholar 

13. Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory, New York.

    Google Scholar 

14. Sakai, Y., Kazarimoto, T. and Tani, Y. 1992. The orotidine-5′-phosphate decarboxylase gene (URA3) of a methylotrophic yeast, Candida boidinii: nudeotide sequence and its expression in Bscherichia coli. J. Ferment. Biotechnol. 73: 255–260.

    Article  CAS  Google Scholar 

15. Sakai, Y. and Tani, Y. 1992. Directed mutagenesis in an asporogenous methylotrophic yeast: cloning, sequencing, and one-step gene disruption of the 3-isopropylmalate dehydrogenase gene (LEU2) of Candida boidinii to derive doubly auxotrophic marker strains. J. Bacteriol. 174: 5988–5993.

    Article  CAS  Google Scholar 

16. Sakai, Y., Goh, T.K. and Tani, Y. 1993. High-frequency transformation of a methylotropic yeast, Candida boidinii, with autonomously replicating plasmids which are also functional in Saccharomyces cerevisiae. J. Bacteriol. 175: 3556–3562.

    Article  CAS  Google Scholar 

17. Hanahan, D. 1985. Techniques for transformation of E. coli, p. 109–136. In: DNA Cloning: A Practical Approach. Glover, D. M. (Ed.). IRL Press Ltd., Oxford.

    Google Scholar 

18. Sakai, Y. and Tani, Y. 1988. Simple purification and improvement of stability of alcohol oxidase from a methanol yeast, Candida boidinii S2, by forming an enzyme-azide complex. Agric. Biol. Chem. 52: 227–233.

    CAS  Google Scholar 

19. Yonehara, T. and Tani, Y. 1986. Comparative studies on ATP production from adenosine by a methanol yeast. Agric. Biol. Chem. 50: 899–905.

    CAS  Google Scholar 

20. Shimizu, S., Miyata, K., Tani, Y. and Ogata, K. 1972. A new process for the production of coenzyme A and its intermediates with a microorganism. Biochem. Biophys. Acta 279: 583–586.

    Article  CAS  Google Scholar 

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