Supplementary Figure 6: Characterisation of Fn-Lam511 ES cells. | Nature Cell Biology

Supplementary Figure 6: Characterisation of Fn-Lam511 ES cells.

From: The ability of inner-cell-mass cells to self-renew as embryonic stem cells is acquired following epiblast specification

Supplementary Figure 6

(A) Alkaline phosphatase staining of ESC colonies and PrE-like cells at day 7 after single ESC derivation in 2i-LIF on Fn-Lam511. (B, C) Immunofluorescence staining of ESC colonies and PrE-like cells at days 3 and 6 (B) and at day 7 (C) from single cell ESC derivation in 2i-LIF on Fn-Lam511. (D) Phase contrast images of the first 5 clonal ESC lines at passage 4 originally derived on either Fn-Lam511 (FL), Fibronectin (F) and Laminin511 (L). After manually picking and dissociating primary single cell ESC colonies at day 7, ESC lines were expanded in 2i-LIF on gelatin. Two of the FL lines were randomly selected (FL4 and FL11) and successfully tested for germline transmission. (E) PrE-like cells obtained after single cell ESC derivation in 2i-LIF on Fn-Lam511 of embryos cultured in the presence of FGF2 for 3 days from the 8-cell stage.

Back to article page