Supplementary Figure 5: Secondary structural modeling of LINK-A-protein and –lipid interactions. | Nature Cell Biology

Supplementary Figure 5: Secondary structural modeling of LINK-A-protein and –lipid interactions.

From: The LINK-A lncRNA interacts with PtdIns(3,4,5)P3 to hyperactivate AKT and confer resistance to AKT inhibitors

Supplementary Figure 5

(a) Graphic illustration of predicted LINK-A secondary structure and the stem loops corresponding to protein (black dot line circle) and lipid (red dot line circle) binding. (b) Secondary structure of LINK-A (nt. 1,081-1,140) harboring a stem-loop corresponding to PIP3 binding (red dot line circle). (c) List of DIG-labeled wild-type LINK-A and mutant oligonucleotides used in Alpha assay. (d) RNA agarose gel of in vitro transcribed biotinylated LINK-A (wild-type and single nucleotide mutants). (e) Relative retrieval level of MS2-tagged full-length LINK-A or indicated mutants in MS2-TRAP assay detected by RT–qPCR. For e, mean ± s.e.m. were derived from n = 3 independent experiments (n.s. P > 0.05, two-tailed paired Student’s t-test). Unprocessed original scans of all blots and gels with size marker are shown in Supplementary Fig. 9.

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