Supplementary Figure 3: Scf is expressed by bone marrow adipocytes.
From: Bone marrow adipocytes promote the regeneration of stem cells and haematopoiesis by secreting SCF
(a) Confocal imaging showed five consecutive optical images (each 1.5 μm) demonstrating the expression of Scf-GFP in perilipin+ adipocytes from non-irradiated mice (representative images from 3 independent experiments). (b–f) Quantitative RT-PCR analysis of leptin (b), Ob-Rb (c), Adipoq (d), perilipin (e) and FABP4 (f) transcript levels (normalized to β-Actin) in LepR+ stromal cells (LepR+), bone marrow adipocytes (Adip-BM) and intraperitoneal adipocytes (Adip-IP) relative to unfractionated whole bone marrow cells (WBM). The transcript levels in WBM were normalized to 1. All data represent mean ± s.d. from n = 3 mice in 3 independent experiments. One-way ANOVAs with Tukey’s multiple comparisons tests were used to assess statistical significance (∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001). (g) Abundant adipocytes in human tibia bone marrow sections from an 8 year-old donor (representative image from 3 independent experiments).
