Figure 5: SNV in Barrett’s stem cell pedigrees.

(a) Histogram of somatic SNV allele frequency determined from exome sequencing of Barrett’s stem cell pedigrees across the patient study group. (b) Distribution and median per cent overlap among SNVs determined from two independent BESC pedigrees (see Methods section). (c) Comparison of distribution of somatic mutation rates between BESC and GSC across the patient study group (see Methods section). (d) Summary of non-synonymous mutations (NS MUT), interstitial deletions (DEL) and interstitial amplifications (AMP) in Barrett’s stem cells cloned from 12 cases without evidence of high-grade dysplasia. Histograms depict total number of events, whereas specific genes were listed beneath each histogram to highlight genes frequently altered in oesophageal and gastric adenocarcinoma^28,29,37. (e) List of average number of CNV events as amplifications (Amp) or deletions (Del) in Barrett’s and gastric stem cells as well as EAC³⁴. (f) Graph depicting the distribution of BESC and GSC by mutation rate (SNV per Mb) and CNV (interstitial deletions)^50,51.