Figure 3: Activation of YAP target genes is ZEB1 specific and requires YAP.
From: ZEB1 turns into a transcriptional activator by interacting with YAP1 in aggressive cancer types
(a) Expression of indicated genes in MDA-MB231 cells upon transient knockdown with the indicated siRNAs. Reduced expression of indicated YAP target genes upon siRNA-mediated knockdown of ZEB1, YAP or TAZ but not SNAI2 in MDA-MB231 cells. mRNA and protein expression were analysed by qRT–PCR and western blot; n=5. (b) qRT–PCR and western blot analysis of MCF10A cells. The iCTR and iZEB1 cells were treated with doxycycline for 6 days and siRNA-mediated knockdown of YAP or TAZ was performed after 3 days for 72 h; n=4. (c) Depletion of ZEB1, YAP or TAZ reverts TGFβ-induced expression of YAP target genes in MCF10A cells. siRNA-mediated knockdown in WT or TGFβ-treated MCF10 cells was performed for 72 h and cells were analysed by qRT–PCR or western blot; n=6. For a–c, mean±s.e.m. *P=0.01–0.05, **P=0.001–0.01, ***P=0.001–0.0001, ****P<0.0001, NS, not significant, unpaired two-tailed Student’s t-test.
