Figure 3: The Msg superfamily in three Pneumocystis species.

(a) Phylogeny of 384 Msg proteins identified in P. murina (blue squares), P. carinii (pink circles) and P. jirovecii (green diamonds). They are classified into five families of Msg-A, -B, -C, -D and -E, as indicated by the vertical bars on the right side. The Msg-A family is further classified into three subfamilies of Msg-A1 (classical Msg genes), Msg-A2 (Msr genes) and Msg-A3 (other Msg-associated genes). (b) Schematic representations of conserved domains in five Msg families. (c) Sequence logos showing the frequency of amino acid composition in Msg domains. Previously identified Pfam MSG and Pfam Msg2_C domains are included for comparison. Additional information on the Msg ___domain analysis is provided in Supplementary Note 4.