Figure 1: Deletion of megalin results in proteinuria containing LCN2.

(a) Wild-type mice express megalin (red) in the proximal tubule, which we identified with Lotus-Tetragonolobus-Lectin (LTL, green). Note the nearly quantitative overlap of megalin and LTL (yellow-orange). (b) Megalin was deleted with Six2Cre, a driver specific to the nephron. Note LTL stained proximal tubules (green) without staining for megalin (absence of red). (c) Megalin deletion resulted in the appearance of urinary LCN2 (detected with mouse specific anti-LCN2) and. (d) Urinary Tf. Scale bars a,b=50 μm.