Figure 1: Development and validation of CRISPR/Cas9 RNP-mediated genome editing in wheat.
From: Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
(a) The exons of TaGW2 and the target site of gw2-sgRNA in exon 8. The single nucleotide polymorphism in the targeted sequence of TaGW2-A1 and the PAM motif are highlighted in green and red, respectively. The XbaI restriction site is underlined. (b) Mutagenesis frequencies of TaGW2-A1, -B1 and -D1 (induced by gw2-RNPs or pGE-TaGW2) in wheat protoplasts analysed by PCR-RE assay. Mutation bands are indicated by red arrows. WT/D and WT/U indicate wild type PCR amplicons with or without restriction enzyme digestion. (c) Mutagenesis frequencies of TaGW2-A1, -B1 and -D1 in embryos treated with gw2-RNPs or pGE-TaGW2 revealed by deep amplicon sequencing.
