Figure 1: Characterization of NIL1913 and identification of CTB4a. | Nature Communications

Figure 1: Characterization of NIL1913 and identification of CTB4a.

From: Natural variation in CTB4a enhances rice adaptation to cold habitats

Figure 1

(a,b) Panicles (a) and seed setting (b) of KMXBG, Towada and NIL1913 planted under normal conditions (Beijing) and cold stress at a higher-altitude area (CS-HAA, Kunming). Data represent means±s.d. (n=15), **P<0.01, *P<0.05, Student’s t-test. Scale bars, 4 cm. (c,d) Pollen fertilities evaluated by I2–KI staining (c), and the blue stained pollen grains were counted for calculating pollen fertility for samples under CS-HAA (d). Scale bars, 25 μm. Data represent means±s.d. (n=10). (e) Fine mapping of CTB4a. Left, high resolution mapping. Right, phenotypes of homozygous recombinants. Significance was determined by one-way ANOVA. Schematic of the gene structure and allelic variation in CTB4a between KMXBG and Towada indicated by vertical lines at bottom. The presence of the same lowercase letter above the error bar denotes a non-significant difference between the means (P>0.05, Student's t-test). (f) qRT-PCR analysis using total RNA isolated from panicles of NIL1913 and Towada under CS-PT at different time points. Data represent means±s.d. (n=3), **P<0.01, *P<0.05, Student’s t-test.

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