Figure 4: SFP-2 reacts with sulphide to give a turn-on fluorescence response.
From: Selective fluorescent probes for live-cell monitoring of sulphide
(a–c) Fluorescence spectra of the SFP-2 probe (5 μM) in PBS buffer (20 mM, pH 7.0, 1% DMSO) at 37 °C. Excitation: 465 nm, emission: 480–580 nm. The data represent the average of three independent experiments. (a) Incubated with 50 μM Na2S after 5, 15, 30, 45, 60, 75, 90, 120, 150, 180 and 240 min. (b) Incubated with different concentrations of Na2S (5, 10, 20, 40, 60, 80 and 100 μM) for 120 min. (c) Incubated with various thiols at 1 mM (BME, 2-mercaptoethanol; CYS, cysteine; GSH, glutathione) for 180 min. (d) Incubated with 1 μl H2S buffer (bubbling H2S 10 min–saturated solution) at 25 °C from 5 s–20 min.
