Figure 2: An RNA stem–loop is required for frameshifting.
From: Protein-directed ribosomal frameshifting temporally regulates gene expression
(a) Mutations introduced to prevent PRF (SS) or StopGo-mediated co-translational separation at the C-terminus of 2A (LV) or to destabilize the predicted RNA stem–loop (SL). (b) Ribo-Seq RPF densities in reads per million mapped reads (RPM) on WT and mutant virus genomes at 8 h p.i. WT and SS samples are biological repeats of the 8 h p.i. samples in Fig. 1. (c) Ratio of SS and SS-SL RPF distributions after smoothing with a 31-nt running-mean filter. (d) Phasing of RPFs mapping upstream of 2B*, within the 2B/2B* overlap region, and downstream of 2B*. (e) Ratio of downstream to upstream RPF densities in the different viruses divided by the corresponding ratio in SS mutant virus.
