Figure 6: Ribosomal pausing at the EMCV frameshift signal.
From: Protein-directed ribosomal frameshifting temporally regulates gene expression
(a) Mutations introduced into the EMCV PRF signal in reporter plasmid pPS0. (b–e) RNAs derived from AvaII-cut plasmids were translated in WG extract, after 5 min further initiation was halted by the addition of edeine, and aliquots were removed at various times and analysed by SDS–PAGE. Lanes M and C show markers and the expected size of the ribosomal pause product, respectively. Translations were supplemented with 1 μM 2A (b–d) or 2A-mut (e). As well as the full-length product and the transient pausing product, a frameshift product is produced for WT RNA only (c).
