Figure 1: CTIF is localized to aggresomes.
From: Misfolded polypeptides are selectively recognized and transported toward aggresomes by a CED complex
(a) Immunostaining for endogenous CTIF (red) and γ-tubulin (green) in HeLa cells in the presence of either DMSO or MG132. (b,c) Quantification of the change in the size (b) and number (c) of CTIF-containing cytoplasmic bodies. Immunostained images of the cells in a were quantitated. The columns and error bars represent the mean and s.d. of three biological replicates. Two-tailed, equal-sample variance Student’s t-tests were used to calculate the P values. **P<0.01. (d) Immunostaining for endogenous CTIF (red) in HeLa cells treated with the indicated inhibitors for 12 h. (e) Immunostaining for endogenous vimentin (green) and CTIF (red) in HeLa cells. Nuclei were stained with DAPI (blue). Enlarged images of the boxed areas are shown in the lower-right corner of each image. All results are representative of three independent biological replicates (n=3). Scale bar, 10 μm.
