Figure 3: Cyclopamine binding to smoothened receptor.
From: Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
(a) Receptor model is shown as a grey cartoon, cyclopamine as a stick model with green carbons, and the ‘omit’ 2mFo–DFc density map for cyclopamine contoured at 1σ is shown as green wires. Horizontal lines indicate membrane boundaries. (b) Cyclopamine binding pocket is shown in stereo view as a stick model along with 2mFo–DFc density map contoured at 1σ. Cyclopamine binds near to the entrance into a long and narrow cavity inside the receptor. Polar interactions stabilizing the shape of the cavity and cyclopamine binding are shown as grey dashed lines. Viewing angles in (a) and (b) are slightly different.
