Figure 3: CD24 promotes growth of DU145 cells.
(a) CD24 mRNA levels in three human prostate cancer cell lines are displaced as % of glyceraldehyde 3-phosphate dehydrogenase (GAPDH). (b) Efficient gene silencing of the CD24 gene based on the protein (insert, representative images that have been reproduced three times) and RNA levels. (c) ShRNA silencing eliminates cell surface CD24 expression as determined by flow cytometry. Data shown are representative profiles and have been reproduced three times. (d) ShRNA silencing of CD24 reduces the growth rate as measured by the 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide assay. Means and s.d. of the relative cell proliferation are shown on the given days. The study has been reproduced three times. (e) Reduced growth rate of tumour cells based on colony size. Data shown are representative images and have been reproduced three times. (f) ShRNA silencing of CD24 reduced the tumour growth in the Il2rg−/− NOD.SCID (NSG) mice. The mice received a subcutaneous injection of 5 × 106 tumour cells. Data shown are means and s.d. of the tumour volumes. (g) Weights of tumours at day 39. Error bars, s.d. Scr n=14, Sh1, n=9, Sh2 n=9 for f and g. Substantially similar data were obtained when the cells were injected into nu/nu mice.
