Figure 5: STAT5B target genes are transcriptionally upregulated in STAT5B-mutant transduced cells through direct binding to conserved STAT5 binding sites. | Nature Communications

Figure 5: STAT5B target genes are transcriptionally upregulated in STAT5B-mutant transduced cells through direct binding to conserved STAT5 binding sites.

From: Activating mutations of STAT5B and STAT3 in lymphomas derived from γδ-T or NK cells

Figure 5

Relative mRNA expression of IL2Rα (a), BCL-XL (b), BCL2 (c), MIR155HG (d) and, HIF2α (e) is shown in control vector or STAT5B-mut transduced cells obtained by sorting GFP+ cells 10 days post transduction, and then culturing for 5 days and 2 days in culture medium with regular or limiting (25IUml−1) IL2 concentrations, respectively. Means±s.d. of two independent experiments (total combined samples=4) are shown. *P<0.01 compared with WT, Student’s t-test. ChIP-q-PCR results for known STAT5 binding sites are shown for IL2Rα (f), BCL-XL (g), BCL2 (h), MIR155HG (i) and HIF2α (j) in EV, STAT5B-WT or STAT5B-N642H transduced KAI3 cells. Data are mean±s.d. of four replicates. STAT5 pull-down normalized to IgG control as a fold difference compared with STAT5B-WT sample is shown in the y-axis using a log scale. STAT5B consensus sites and their approximate distance to the TSS sites are indicated.

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